- CASA SOFTWARE SPERM PDF
- CASA SOFTWARE SPERM MANUAL
- CASA SOFTWARE SPERM LICENSE
- CASA SOFTWARE SPERM FREE
Permission to use, copy, modify, and distribute this Theriogenology (2006), doi:10.1016/j.theriogenology.2006.10.003.Ĭopyright © 2003 The Regents of the University of California Source software for characterization of zebrafish sperm motility parameters, Wilson-Leedy JG, Ingermann RL, Development of a novel CASA system based on open ForĪll motion parameters measured (except duration), the average CV was <ġ0 %, comparable to values obtained using commercial systems. Semen volume and concentration) was positively correlated to percent motility. Furthermore, relative sperm output (a measure reflecting both
There was significant inter-male variation for VCL, VSL, velocityĪverage path (VAP), percent motility, path character (STR, LIN), and duration Sperm density in the field of view did not affect any measured From 30 to 300 frames/sec,įrame rate significantly affected curvilinear velocity (VCL) and STR (STR), but not linearity (LIN), relative to time. There were decreases in velocity and path straightness To time post-activation and the impact of acquisition conditions upon dataĪnalysis determined. Using this system, the motion of zebrafish ( Danio rerio) sperm was characterized relative Straight line (VSL) algorithm, eliminating inaccurate characterization of highlyĬurved fish sperm paths. In addition, we have improved upon the traditional velocity Improve quality control and enhance the comparative value of data obtainedĪmong laboratories. That it is open source makes possible external validation, should Software ImageJ and is available with documentation at.
CASA SOFTWARE SPERM FREE
This software is a plugin for the free National Institutes of Health Written and validated a free CASA software primarily for analysis of fish
CASA SOFTWARE SPERM MANUAL
Outperforms manual techniques, many investigators rely on non-automatedĪnalysis due to the high cost of commercial options. Novel CASA system based on open source software for characterization ofĪlthough computer assisted sperm analysis (CASA)
CASA SOFTWARE SPERM LICENSE
This plugin is subject to the license agreement Please also visit the following website for further information.
CASA SOFTWARE SPERM PDF
Please review reference below as well as the PDF manual provided:ĬASA instructions.pdf. Of a stack and will output commonly reported parameters of sperm motionĪs well as the percentage of sperm moving. This plugin will analyze video of sperm in the form
Tested using ImageJ 1.36 with Java 1.3.1_13 on This plugin is subject to the license agreementĭownload CASA_.java to the plugins folder, compile and run. Included in CASA_.java, and is released under the GNU These findings suggest that, in the high and occupational nickel exposure environments, the contribution of nickel toxicity to the males who wish to preserve their fertility is worthy of careful evaluation.Computer Assisted Sperm Analyzer Computer Assisted Sperm Analyzer Taken together, these results revealed that nickel could compromise sperm functions by interfering with Ca 2+ signaling and inducing excessive oxidative stress. Consistently, eliminating excess ROS by N-acetyl- L-cysteine or tocopherol significantly alleviated nickel-impaired sperm motility. Besides, reactive oxygen species (ROS), superoxide, and malondialdehyde levels were increased in human sperm after exposure to different concentrations of NiCl 2. Mechanically, NiCl 2 significantly inhibited the basal intracellular Ca 2+ signaling. In addition, sperm hyperactivation and the ability of human sperm to penetrate a viscous medium were found to be compromised after nickel exposure. We found that NiCl 2 (10, 50, and 250 μM) impaired sperm total motility and progressive motility in a dose- and time-dependent manner. In this study, the toxicity of nickel on human sperm and the underlying mechanisms were evaluated in vitro. However, how nickel affects human reproduction is still unclear. As one of the main environmental pollutants and occupational hazards, nickel has been reported to have mutagenic, carcinogenic, and teratogenic properties, as well as reproductive toxicity.
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